DOT1L recombinant monoclonal antibody, clone R01-7F6
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human DOT1L.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human DOT1L.
Theoretical MW (kDa)
Calculated MW: 165 k
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Hela, A549, HL-60 lysates with DOT1L recombinant monoclonal antibody, clone R01-7F6 (Cat # RAB01421).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded mouse testis using KMT4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Gene Info — DOT1L
Entrez GeneID
84444Protein Accession#
Q8TEK3Gene Name
DOT1L
Gene Alias
DKFZp586P1823, DOT1, KIAA1814, KMT4
Gene Description
DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Omim ID
607375Gene Ontology
HyperlinkGene Summary
O
Other Designations
DOT1-like, histone H3 methyltransferase|histone methyltransferase DOT1L
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