LMAN1 recombinant monoclonal antibody, clone R03-5C3
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More Files
- More Functions
Western Blot
Western Blot analysis of Hela, A549, HL-60, U251, U87-MG lysates with LMAN1 recombinant monoclonal antibody, clone R03-5C3 (Cat # RAB01417).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using LMAN1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LMAN1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human LMAN1.
Theoretical MW (kDa)
Calculated MW: 58 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Hela, A549, HL-60, U251, U87-MG lysates with LMAN1 recombinant monoclonal antibody, clone R03-5C3 (Cat # RAB01417).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using LMAN1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Gene Info — LMAN1
Entrez GeneID
3998Protein Accession#
P49257Gene Name
LMAN1
Gene Alias
ERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58
Gene Description
lectin, mannose-binding, 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq
Other Designations
coagulation factor V-factor VIII combined deficiency|endoplasmic reticulum-golgi intermediate compartment protein 53|intracellular mannose specific lectin
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Interactome
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Disease
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