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Immunohistochemistry

Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab). Antigen retreival was achieved by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation was carried out at 4 ug/mL for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3′-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/mL.

Entrez GeneID

2064

Protein Accession#

P04626

Gene Name

ERBB2

Gene Alias

CD340, HER-2, HER-2/neu, HER2, NEU, NGL, TKR1

Gene Description

v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Omim ID

137215 137800 164870 211980

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq

Other Designations

c-erb B2/neu protein|erbB-2|herstatin|neuroblastoma/glioblastoma derived oncogene homolog|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)