SLC7A5 recombinant monoclonal antibody, clone EP3-1
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More Files
- More Functions
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 at 10 ug/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
Flow Cytometry
Flow-cytometry were stained with anti-unknown specificity antibody at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD98.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. CD98-reactive recombinant Fab fragments were retrieved by epitope peptide panning.
Reactivity
Human
Specificity
Shown to bind to epitopes of sequences; LLHGDFHAFSAG-GGGC-NH2, LLHGDFH-GGGC-HN2, and FHAFSAG-GGGC-NH2, but showed no reactivity to live HeLa cells by IF.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG, Kappa
Recommend Usage
Flow cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Applications
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EP3-1 at 10 ug/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.Flow Cytometry
Flow-cytometry were stained with anti-unknown specificity antibody at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — SLC7A5
Entrez GeneID
8140Protein Accession#
Q01650Gene Name
SLC7A5
Gene Alias
4F2LC, CD98, D16S469E, E16, LAT1, MPE16, hLAT1
Gene Description
solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Omim ID
600182Gene Ontology
HyperlinkGene Summary
y+ system)
Other Designations
4F2 light chain|CD98 light chain|L-type amino acid transporter 1|integral membrane protein E16|large neutral amino acids transporter 1|large neutral amino acids transporter small subunit 1|sodium-independent neutral amino acid transporter LAT1
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Interactome
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Disease
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