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Last updated: 2022/5/22

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AFP monoclonal antibody, clone AF5 

  • Catalog # : RAB01092
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  • Specification
  • Product Description:
  • Recombinant rabbit monoclonal antibody raised against human AFP.
  • Immunogen:
  • Originate antibody is raised against AFP from human amniotic fluid.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to human AFP.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purified
  • Isotype:
  • Rabbit IgG, kappa
  • Recommend Usage:
  • Flow Cytometry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.02% Proclin 300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of AFP monoclonal antibody, clone AF5 (Cat # RAB01092).
    HepG2 cell lysate (35 ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit antiby (Cat # RAB01092) at 1 ug/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Alpha-fetoprotein is 68.7kDa, though this protein is also glycosylated (Uniprot ID: P02771). Cat # RAB01092 successfully detected human AFP in HepG2 cell lysates.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluoresence staining of fixed HepG2 cells with AFP monoclonal antibody, clone AF5 (Cat # RAB01092).
    Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton stained with the chimeric rabbit antibody (Cat # RAB01092) at 10 ug/mL for 1h followed by Alexa Fluor®488 secondary antibody (1 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Cat # RAB01092, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor®488 secondary antibody.
  • Flow Cytometry
  • Flow Cytometry
  • Flow-cytometry using AFP monoclonal antibody, clone AF5 (Cat # RAB01092)
    HepG2 cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric antibody (Cat # RAB01092, blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/mL and cells analyzed on a FACSCanto flow-cytometer.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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  • Gene Information
  • Entrez GeneID:
  • 174
  • Gene Name:
  • AFP
  • Gene Alias:
  • FETA,HPAFP
  • Gene Description:
  • alpha-fetoprotein
  • Gene Summary:
  • This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000160480,alpha-1-fetoprotein,alpha-fetoglobulin
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