HLA class II monoclonal antibody, clone F3.3
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of HLA class II monoclonal antibody, clone F3.3 (Cat # RAB01090).
Human spleen (A), human tonsil (B) and Daudi cell lysate (C) samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit antibody (Cat # RAB01090) at 0.01 ug/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for HLA II DR is ~30 kDa, depending upon the exact DR isoform expressed (c.f. Klitz et al, PMID: 12974796). Cat # RAB01090 successfully detected human HLA class II histocompatibility antigen DR in human spleen and tonsil lysate as well as Daudi cell lysate.
Flow Cytometry
Flow-cytometry using HLA class II monoclonal antibody, clone F3.3 (Cat # RAB01090).
Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of F3.3 (Cat # RAB01090, panel B) at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Flow Cytometry
Flow-cytometry using HLA class II monoclonal antibody, clone F3.3 (Cat # RAB01090).
Daudi cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric antibody (Cat # RAB01090, blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/mL and cells analyzed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HLA class II.
Antibody Species
Rabbit
Reactivity
Human
Specificity
This antibody reacts to class II HLA molecules.
Form
Liquid
Purification
Protein A affinity purified
Isotype
Rabbit IgG, kappa
Recommend Usage
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot analysis of HLA class II monoclonal antibody, clone F3.3 (Cat # RAB01090).
Human spleen (A), human tonsil (B) and Daudi cell lysate (C) samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit antibody (Cat # RAB01090) at 0.01 ug/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for HLA II DR is ~30 kDa, depending upon the exact DR isoform expressed (c.f. Klitz et al, PMID: 12974796). Cat # RAB01090 successfully detected human HLA class II histocompatibility antigen DR in human spleen and tonsil lysate as well as Daudi cell lysate.Flow Cytometry
Flow-cytometry using HLA class II monoclonal antibody, clone F3.3 (Cat # RAB01090).
Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of F3.3 (Cat # RAB01090, panel B) at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.Flow Cytometry
Flow-cytometry using HLA class II monoclonal antibody, clone F3.3 (Cat # RAB01090).
Daudi cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric antibody (Cat # RAB01090, blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/mL and cells analyzed on a FACSCanto flow-cytometer.
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