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Last updated: 2022/5/22

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HLA class II monoclonal antibody, clone F3.3 

  • Catalog # : RAB01090
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  • Specification
  • Product Description:
  • Recombinant rabbit monoclonal antibody raised against human HLA class II.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to class II HLA molecules.
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purified
  • Isotype:
  • Rabbit IgG, kappa
  • Recommend Usage:
  • Flow Cytometry
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.02% Proclin 300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of HLA class II monoclonal antibody, clone F3.3 (Cat # RAB01090).
    Human spleen (A), human tonsil (B) and Daudi cell lysate (C) samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit antibody (Cat # RAB01090) at 0.01 ug/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for HLA II DR is ~30 kDa, depending upon the exact DR isoform expressed (c.f. Klitz et al, PMID: 12974796). Cat # RAB01090 successfully detected human HLA class II histocompatibility antigen DR in human spleen and tonsil lysate as well as Daudi cell lysate.
  • Flow Cytometry
  • Flow Cytometry
  • Flow-cytometry using HLA class II monoclonal antibody, clone F3.3 (Cat # RAB01090).
    Daudi cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric antibody (Cat # RAB01090, blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/mL and cells analyzed on a FACSCanto flow-cytometer.
  • Flow Cytometry
  • Flow Cytometry
  • Flow-cytometry using HLA class II monoclonal antibody, clone F3.3 (Cat # RAB01090).
    Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of F3.3 (Cat # RAB01090, panel B) at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
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