SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Flow Cytometry (Transfected cell)
Flow cytometry (transfected cell) analysis of 293T-SARS-CoV-2 spike cells were treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J). This antibody efficiently bound to 293T- SARS-CoV-2 spike cells.
Inhibition Assay
Protein receptor-binding inhibition assay of spike-His protein was treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J), and then added into 293T-ACE2 cells. Data were analyzed by flow cytometer. Binding affinity of spike-His protein to ACE2 is totally blocked by this antibody.
Neutralization
Neutralization analysis of pseudovirus was treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J), and then added into 293T-ACE2 cells. Luciferase activity was measured 48 hours post infection.
Neutralization
Neutralization analysis of variant pseudovirus was treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J), and then added into 293T-ACE2 cells. Luciferase activity was measured 48 hour post infection.
Neutralization
Cytopathic effect (CPE) based live virus neutralization analysis with human IgG1 isotype control and SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J). The antibody solutions (100 uL) were mixed in 1:1 (v/v) with suspension containing 200 TCID50 of SARS-CoV-2 virus.
Antibody Kinetics
Antibody kinetics with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J) and data were analyzed by biacore surface plasmon resonance.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis coated mucin (100 ug/mL) with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J).
Neutralization
In in vivo neutralization analysis, hamsters received 2 doses of SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J) by intraperitoneal injection and then were infected with 3 doses pseudovirus through intanasal injection. Lung tissues were collected one day post last dose pseudovirus treatment. Luciferase gene expression level was detected by QPCR.
Neutralization
In in vivo nasal prophylaxis neutralization analysis. Hamsters were infected with either B.1.1.28.1 (upper panel) or B.1.617.2 (lower panel) variant spike pseudovirus 2 hours after receiving SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J). Lung tissues were collected one day after the last dose of pseudovirus treatment. Luciferase gene expression level was detected by qPCR.
-
Specification
Product Description
Human recombinant monoclonal antibody raised against SARS-CoV-2 S.
Antibody Species
Human
Immunogen
SARS-CoV-2 S recombinant protein
Reactivity
SARS-CoV-2
Specificity
Reacts with RBD domain of spike protein of SARS-CoV-2, including B.1.1.7, B.1.351 and B.1.1.28.1 variants.
Form
Liquid
Purification
Protein A sepharose
Isotype
Human IgG1
Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Flow Cytometry (Transfected cell)
Flow cytometry (transfected cell) analysis of 293T-SARS-CoV-2 spike cells were treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J). This antibody efficiently bound to 293T- SARS-CoV-2 spike cells.Inhibition Assay
Protein receptor-binding inhibition assay of spike-His protein was treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J), and then added into 293T-ACE2 cells. Data were analyzed by flow cytometer. Binding affinity of spike-His protein to ACE2 is totally blocked by this antibody.Neutralization
Neutralization analysis of pseudovirus was treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J), and then added into 293T-ACE2 cells. Luciferase activity was measured 48 hours post infection.Neutralization
Neutralization analysis of variant pseudovirus was treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J), and then added into 293T-ACE2 cells. Luciferase activity was measured 48 hour post infection.Neutralization
Cytopathic effect (CPE) based live virus neutralization analysis with human IgG1 isotype control and SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J). The antibody solutions (100 uL) were mixed in 1:1 (v/v) with suspension containing 200 TCID50 of SARS-CoV-2 virus.Antibody Kinetics
Antibody kinetics with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J) and data were analyzed by biacore surface plasmon resonance.Epitope Mapping
SARS-CoV-2 S1+S2 domain (residues 16-1213): positive
SASRS-CoV-2 S1 domain (residues 1-674): positive
SARS-CoV-2 receptor binding domain (residues 319-541): positive
SARS-CoV-2 core domain + receptor binding subdomain (residues 387-516): positive
SARS-CoV-2 receptor-binding motif (residues 438-505): positive
Data were analyzed by indirect ELISA.Enzyme-linked Immunoabsorbent Assay
ELISA analysis coated mucin (100 ug/mL) with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J).Neutralization
In in vivo neutralization analysis, hamsters received 2 doses of SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J) by intraperitoneal injection and then were infected with 3 doses pseudovirus through intanasal injection. Lung tissues were collected one day post last dose pseudovirus treatment. Luciferase gene expression level was detected by QPCR.Neutralization
In in vivo nasal prophylaxis neutralization analysis. Hamsters were infected with either B.1.1.28.1 (upper panel) or B.1.617.2 (lower panel) variant spike pseudovirus 2 hours after receiving SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J). Lung tissues were collected one day after the last dose of pseudovirus treatment. Luciferase gene expression level was detected by qPCR.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com