SARS-CoV-2 Beta Variant S recombinant monoclonal antibody
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Specification
Product Description
Human recombinant monoclonal antibody raised against SARS-CoV-2 Beta Variant S.
Antibody Species
Human
Immunogen
Recombinant protein corresponding to SARS-CoV-2 Variant B.1.351 S.
Reactivity
SARS-CoV-2
Specificity
Reacts with RBD domain of spike protein of SARS-CoV-2, including South Africa variant (B.1.351) and UK variant (B.1.1.7).
Form
Liquid
Preparation Method
This antibody is a CHO expressed recombinant antibody. It has the characteristic human Fc domain.
Purification
Affinity chromatography
Isotype
Human IgG
Recommend Usage
ELISA
Neutralization Assay
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (40% glycerol)
Storage Instruction
Store at 4°C is stable for 6 months from the date of shipment. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result Data
Pseudoviral particle (PP) infection assay challenged by SARS-CoV-2 Variant B.1.351 S recombinant monoclonal antibody (Cat # RAB01067).
HEK293-ACE2 cells infected with SARS-CoV-2 pseudoviral particles under various amounts of SARS-CoV-2 Variant B.1.351 S recombinant monoclonal antibody (Cat # RAB01067), with IC50 of 42.8 ng/mL (SARS-CoV-2 Pseudoviral Particles, D614G) and 45 ng/mL (SARS-CoV-2 Pseudoviral Particles, South Africa Variant).
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Applications
Enzyme-linked Immunoabsorbent Assay
Titration curves of SARS-CoV-2 Variant B.1.351 S recombinant monoclonal antibody (Cat # RAB01067). 96-well corning ELISA plate was coated with S (SARS-CoV-2 Variant B.1.351) RBD Recombinant Protein (Cat # P6703) at a concentration of 2 ug/mL, and with a EC50 of 12.19 ng/mL.Neutralization
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