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Last updated: 2021/4/11

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SARS-CoV-2 N recombinant monoclonal antibody, clone 85E9 

  • Catalog # : RAB01065
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  • Specification
  • Product Description:
  • Recombinant rabbit monoclonal antibody raised against SARS-CoV-2 N.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of SARS-CoV-2 N.
  • Host:
  • Rabbit
  • Reactivity:
  • SARS-CoV, SARS-CoV-2
  • Specificity:
  • Highly-sensitive to both SARS-CoV-2 and SARS-CoV nucleoprotein.
  • Form:
  • Liquid
  • Preparation Method:
  • 85E9 is derived from a Fab phage display library made from a rabbit immunized with synthetic peptide specific to the C-terminus of SARS-CoV-2 nucleocapsid protein. Libraries were selected on N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and Fab sup were screened on SARS-CoV-2 NP and SARS-CoV (2003) NP by ELISA. Positive clones were cloned into a bi-cistronic IgG vector and produced in HEK293 cells. The antibodies were purified from a Protein A column.
  • Purification:
  • Protein A purification
  • Concentration:
  • 0.5 mg/mL
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 1X PBS (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • 75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), 84D7 (Cat # RAB01057), 85C1 (Cat # RAB01059), 85C10 (Cat # RAB01061), 85B4 (Cat # RAB01063), and 85E9 (Cat # RAB01065) can be directly coated to the ELISA wells as a capture antibody and the other antibody (e.g. biotinylated) [75G5a (Cat # RAB01054), 84C4a (Cat # RAB01056), 84D7 (Cat # RAB01058), 85C1 (Cat # RAB01060), 85C10 (Cat # RAB01062), 85B4 (Cat # RAB01064), and 85E9 (Cat # RAB01066)] can be used as a detecting antibody with StreptAvidin-HRP. For highest sensitivity, we recommend pairing unconjugated clone 75G5a (Cat # RAB01053), immobilized on the ELISA plate to capture COVID-19 NP antigen, with SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) for detection using high-sensitivity streptavidin-HRP (Pierce #21130).
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL SARS-CoV-2 N recombinant monoclonal antibody, clone 85E9 (Cat # RAB01065).
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and SARS-CoV NP at 1 ug/mL. Purified SARS-CoV-2 N recombinant monoclonal antibody, clone 85E9 (Cat # RAB01065) was serially diluted 1:2 starting at 1 ug/mL. Rabbit monoclonal antibody 85E9 shows very strong and specific binding to both COVID-19 NP antigen and recognizes SARS-CoV NP antigen.
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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