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Last updated: 2020/8/9

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SARS-CoV-2 S ECD recombinant monoclonal antibody, clone BHB05 Coronavirus

  • Catalog # : RAB01049
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  • Specification
  • Product Description:
  • Recombinant human monoclonal antibody raised against SARS-CoV-2 S ECD.
  • Immunogen:
  • Natural SARS-CoV-2.
  • Host:
  • Human
  • Reactivity:
  • Viruses
  • Specificity:
  • This antibody detects endogenous levels of S-ECD protein.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Purity:
  • > 95% (by SDS-PAGE).
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (1:80000)
    Neutralization
    Western Blot (1:3000-1:4000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2.
  • Storage Instruction:
  • Store at 4°C for short term. For long term storage, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Antibody Species:
  • Human
  • Matched Pair:
  • RAB01045 with RAB01049; RAB01046 with RAB01049
  • Applications
  • Neutralization
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western Blot analysis of Lane 1: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) 1 ug and Lane 2: S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 1 ug. Primary antibody: SARS-CoV-2 S ECD recombinant monoclonal antibody, clone BHB05 (Cat # RAB01049) (1:4000). Secondary antibody: Goat anti-human IgG+HRP (1:5000). Exposure time: 120 s.
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • An ELISA was performed using 1. Coating original concentration: 2 ug/mL, 100 uL/hole sample is S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) and S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 2. Primary antibody: SARS-CoV-2 S ECD recombinant monoclonal antibody, clone BHB05 (Cat # RAB01049) 3. Secondary antibody: Goat anti-human IgG+HRP (1:10000) 4. Develop: 15 min, 100 uL/hole 5. Stop: stop buffer 50 uL/hole 6. ELISA result are shown in the figure.
  • Application Image
  • Neutralization
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
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