SARS-CoV-2 S ECD recombinant human antibody, clone BHB05
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More Files
- More Functions
Western Blot (Recombinant protein)
Western Blot analysis of Lane 1: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) 1 ug and Lane 2: S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 1 ug. Primary antibody: SARS-CoV-2 S ECD recombinant human antibody, clone BHB05 (Cat # RAB01049) (1:4000). Secondary antibody: Goat anti-human IgG+HRP (1:5000). Exposure time: 120 s.
Enzyme-linked Immunoabsorbent Assay
An ELISA was performed using 1. Coating original concentration: 2 ug/mL, 100 uL/hole sample is S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) and S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 2. Primary antibody: SARS-CoV-2 S ECD recombinant human antibody, clone BHB05 (Cat # RAB01049) 3. Secondary antibody: Goat anti-human IgG+HRP (1:10000) 4. Develop: 15 min, 100 uL/hole 5. Stop: stop buffer 50 uL/hole 6. ELISA result are shown in the figure.
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Specification
Product Description
Human recombinant monoclonal antibody raised against SARS-CoV-2 S ECD.
Antibody Species
Human
Source / Expression System
293F
Immunogen
Natural SARS-CoV-2
Reactivity
SARS-CoV-2
Specificity
This antibody detects endogenous levels of S-ECD protein.
Form
Liquid
Purification
Protein A purification
Purity
> 95% (by SDS-PAGE).
Isotype
IgG
Recommend Usage
ELISA (1:80000)
Neutralization
Western Blot (1:3000-1:4000)
The optimal working dilution should be determined by the end user.Matched Pair
RAB01045 with RAB01049; RAB01046 with RAB01049
Storage Buffer
In PBS, pH 7.2
Storage Instruction
Store at 4°C for short term. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western Blot analysis of Lane 1: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) 1 ug and Lane 2: S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 1 ug. Primary antibody: SARS-CoV-2 S ECD recombinant human antibody, clone BHB05 (Cat # RAB01049) (1:4000). Secondary antibody: Goat anti-human IgG+HRP (1:5000). Exposure time: 120 s.Enzyme-linked Immunoabsorbent Assay
An ELISA was performed using 1. Coating original concentration: 2 ug/mL, 100 uL/hole sample is S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6661) and S (SARS-CoV-2) ECD Recombinant Protein (Cat # P6662) 2. Primary antibody: SARS-CoV-2 S ECD recombinant human antibody, clone BHB05 (Cat # RAB01049) 3. Secondary antibody: Goat anti-human IgG+HRP (1:10000) 4. Develop: 15 min, 100 uL/hole 5. Stop: stop buffer 50 uL/hole 6. ELISA result are shown in the figure.Neutralization
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