Recombinant human antibody raised against partial recombinant SARS-CoV-2 S RBD.
Immunogen:
Original antibody is raised against recombinant protein corresponding to SARS-CoV-2 S1.
Host:
Human
Reactivity:
SARS-CoV-2
Form:
Liquid
Purification:
Protein A purification
Purity:
> 95% by HPLC & SDS-PAGE.
Isotype:
IgG
Quality Control Testing:
Testing results of lateral flow immunoassay
Testing S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6630) with Cat # RAB01036 - Cat # RAB01037 in lateral flow mmunoassay. The minimum detectable value for SARS-CoV-2 S RBD protein and antibody pair (Cat # RAB01036- Cat # RAB01037) is 1 ug/mL. Result zone of the Testing Strip indicates SARS-CoV-2 S RBD line (T line) and control line (C line).
Recommend Usage:
ELISA Lateral-Flow Immunochromatographic Assay The optimal working dilution should be determined by the end user.
Storage Buffer:
In 0.01 M PBS.
Storage Instruction:
Store at -20°C for long term storage. Aliquot to avoid repeated freezing and thawing.
1. Conjugate pad: Precoated with Cat # RAB01037 (SARS-CoV-2 S RBD recombinant human antibody, clone 1A1, need to conjugate with colloidal gold first) and Chicken IgY (need to conjugate with colloidal gold first) 2. T line: Cat # RAB01036 (SARS-CoV-2 S RBD recombinant human antibody, clone 2C1) 3. C line: Rabbit anti-chicken IgY Lane 1: 1x PBS was added at the sample hole (Negative) Lane 2: Lysis buffer was added at the sample hole (Negative) Lane 3: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6630, 0.45 mg/mL) with dilution at 1:100 (Positive) Lane 4: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6630, 0.45 mg/mL) with dilution at 1:500 (Positive) Lane 5: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6630, 0.45 mg/mL) with dilution at 1:1000 (Weak Positive) The minimum detectable value is 0.45 ug/mL for this lot.