SARS-CoV-2 S RBD recombinant human antibody, clone 2C1
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Specification
Product Description
Human recombinant monoclonal antibody raised against partial recombinant SARS-CoV-2 S RBD.
Antibody Species
Human
Source / Expression System
HEK293
Immunogen
Original antibody is raised against recombinant protein corresponding to SARS-CoV-2 S1.
Reactivity
SARS-CoV-2
Form
Liquid
Purification
Protein A purification
Purity
> 95% by HPLC & SDS-PAGE.
Isotype
IgG
Quality Control Testing
Testing results of lateral flow immunoassay
Testing S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6630) with Cat # RAB01036 - Cat # RAB01037 in lateral flow mmunoassay. The minimum detectable value for SARS-CoV-2 S RBD protein and antibody pair (Cat # RAB01036- Cat # RAB01037) is 1 ug/mL. Result zone of the Testing Strip indicates SARS-CoV-2 S RBD line (T line) and control line (C line).
Recommend Usage
ELISA
Lateral-Flow Immunochromatographic Assay
The optimal working dilution should be determined by the end user.Matched Pair
Capture Ab: RAB01036
Detection Ab: RAB01037Storage Buffer
In 0.01 M PBS
Storage Instruction
Store at -20°C for long term storage.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Lateral-Flow Immunochromatographic Assay
1. Conjugate pad: Precoated with Cat # RAB01037 (SARS-CoV-2 S RBD recombinant human antibody, clone 1A1, need to conjugate with colloidal gold first) and Chicken IgY (need to conjugate with colloidal gold first)
2. T line: Cat # RAB01036 (SARS-CoV-2 S RBD recombinant human antibody, clone 2C1)
3. C line: Rabbit anti-chicken IgY
Lane 1: 1x PBS was added at the sample hole (Negative)
Lane 2: Lysis buffer was added at the sample hole (Negative)
Lane 3: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6630, 0.45 mg/mL) with dilution at 1:100 (Positive)
Lane 4: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6630, 0.45 mg/mL) with dilution at 1:500 (Positive)
Lane 5: S1 (SARS-CoV-2) RBD Recombinant Protein (Cat # P6630, 0.45 mg/mL) with dilution at 1:1000 (Weak Positive)
The minimum detectable value is 0.45 ug/mL for this lot.
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