GPC3 recombinant monoclonal antibody, clone GPC3/1534R
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human fetal liver.
Immunofluorescence
Immunofluorescence staining of MeOH-fixed Human HepG2 cells labeling Glypican-3. This antibody followed by Goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
Flow Cytometry
Flow cytometric analysis of MeOH-fixed HepG2 cells. This antibody followed by goat anti-rabbit IgG-CF488 (Blue). Isotype Control (Red).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against full length human GPC3.
Antibody Species
Rabbit
Immunogen
Recombinant protein corresponding to full-length human GPC3.
Reactivity
Human, Rat
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1-2 ug/million cells)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
Immunofluorescence (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 1 mg/mL PBS
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human fetal liver.Immunofluorescence
Immunofluorescence staining of MeOH-fixed Human HepG2 cells labeling Glypican-3. This antibody followed by Goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).Flow Cytometry
Flow cytometric analysis of MeOH-fixed HepG2 cells. This antibody followed by goat anti-rabbit IgG-CF488 (Blue). Isotype Control (Red). -
Gene Info — GPC3
Entrez GeneID
2719Protein Accession#
P08729Gene Name
GPC3
Gene Alias
DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1
Gene Description
glypican 3
Gene Ontology
HyperlinkGene Summary
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000024058|OTTHUMP00000062492|glypican proteoglycan 3
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