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CD86 recombinant monoclonal antibody, clone C86/2160R 

  • Catalog # : RAB00670
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  • Specification
  • Product Description:
  • Recombinant rabbit monoclonal antibody raised against full length human CD86.
  • Immunogen:
  • Recombinant protein corresponding to full-length human CD86.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (2-4 ug/mL for coating)
    Flow Cytometry (1-2 ug/million cells)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
    Immunofluorescence (1-2 ug/mL)
    Western Blot (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 1 mg/mL PBS.
  • Storage Instruction:
  • Store at -20 to -80°C.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (cell lysate) analysis of Ramos cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human colon.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of Raji cells. Goat anti-rabbit IgG conjugated to CF488 (Green) ; Nuclei are stained with Reddot.
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Ramos cells. This antibody followed by goat anti-mouse IgG-CF488 (Blue). Isotype Control (Red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 942
  • Protein Accession#:
  • P4208
  • Gene Name:
  • CD86
  • Gene Alias:
  • B7-2,B70,CD28LG2,LAB72,MGC34413
  • Gene Description:
  • CD86 molecule
  • Gene Summary:
  • This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq
  • Other Designations:
  • B-lymphocyte activation antigen B7-2,B7-2 antigen,CD28 antigen ligand 2,CD86 antigen,CD86 antigen (CD28 antigen ligand 2, B7-2 antigen),CTLA-4 counter-receptor B7.2
  • Interactome
  • Interactome
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