Product Browser

Last updated: 2017/6/18

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

WT1 recombinant monoclonal antibody, clone WT1/1434R

  • Catalog # : RAB00292
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Recombinant rabbit monoclonal antibody raised against human WT1.
  • Immunogen:
  • Original antibody is raised against recombinant protein corresponding to full length human WT1.
  • Reactivity:
  • Human
  • Specificity:
  • Recognizes a 47-55kDa tumor suppressor protein which identified as WT1 protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG, kappa
  • Recommend Usage:
  • Flow Cytometry (0.5-1 ug/million cells)
    Immunofluorescence (0.5-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS.
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Antibody Species:
  • Rabbit
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with WT1 recombinant monoclonal antibody, clone WT1/1434R (Cat # RAB00292).
  • Immunofluorescence
  • Flow Cytometry
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 7490
  • Gene Name:
  • WT1
  • Gene Alias:
  • GUD,WAGR,WIT-2,WT33
  • Gene Description:
  • Wilms tumor 1
  • Gene Summary:
  • This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
  • Other Designations:
  • -
  • RSS
  • YouTube
  • Linkedin
  • Facebook