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PRMT3 recombinant monoclonal antibody

  • Catalog # : RAB00246
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  • Specification
  • Product Description:
  • Recombinant mouse monoclonal antibody raised against human PRMT3.
  • Immunogen:
  • Original antibody is raised against recombinant protein corresponding to amino acids 211-531 of human PRMT3.
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (3-5 ug x 106 cells)
    Immunocytochemistry (2 ug/mL)
    Immunofluorescence (2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20)
    Immunoprecipitation (2 ug)
    Western Blot (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Antibody Species:
  • Mouse
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: T47D, Lane 2: A549, Lane 3: U2OS, Lane 4: K562, Lane 5: HeLa, Lane 6: Raji, Lane 7: HEK-MSR, Lane 8: MCF7, Lane 9: MDA-MB-231, Lane 10: LNCaP and Lane 11: HepG2 cell lysates with PRMT3 recombinant monoclonal antibody (Cat # RAB00246).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma (A) and human breast carcinoma (B) tissues (right) with PRMT3 recombinant monoclonal antibody (Cat # RAB00246) compared to a negative control without primary antibody respectively (left).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of A549 cells with PRMT3 recombinant monoclonal antibody (Cat # RAB00246). Panel a: PRMT3 (Green). Panel b: nuclei (Blue). Panel c: F-actin (Red). Panel d: merge. Panel e: no primary antibody control.
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation analysis of HEK293 cells with PRMT3 recombinant monoclonal antibody (Cat # RAB00246). Lane 1: PRMT3 input. Lane 2: PRMT3 IP.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF7 cells with PRMT3 recombinant monoclonal antibody (Cat # RAB00246) (Red histogram). Yellow histogram: mouse isotype control. Purple histogram: unstained control cells. Green histogram: no primary antibody control.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Gene Information
  • Gene Name:
  • PRMT3
  • Gene Alias:
  • HRMT1L3
  • Gene Description:
  • protein arginine methyltransferase 3
  • Gene Summary:
  • Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).[supplied by OMIM
  • Other Designations:
  • HMT1 hnRNP methyltransferase-like 3,heterogeneous nuclear ribonucleoprotein methyltransferase-like 3,protein arginine N-methyltransferase 3
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