Recombinant mouse monoclonal antibody raised against human CBX3.
Original antibody is raised against recombinant protein corresponding to amino acids 29-86 of human CBX3.
Human, Mouse, Rat
Flow Cytometry (3-5 ug x 106 cells) Immunocytochemistry (2 ug/mL) Immunofluorescence (1:250) Immunoprecipitation (2 ug) Western Blot (1:2000) The optimal working dilution should be determined by the end user.
In PBS (0.09% sodium azide).
Store at 4°C for up to 1 months. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: A549, Lane 2: PC 3, Lane 3: MCF-7, Lane 4: SH-SY5Y, Lane 5: Caki-1, Lane 6: NIH/3T3, Lane 7: HT-29 cell lysates with CBX3 recombinant monoclonal antibody (Cat # RAB00215).
Western blot analysis of Lane 1: recombinant CBX3, Lane 2: U2OS, Lane 3: HeLa, Lane 4: MEF, Lane 5: NRK, Lane 6: NIH 3T3 cell lysates with CBX3 recombinant monoclonal antibody (Cat # RAB00215).
Immunofluorescent staining of HeLa cell with CBX3 recombinant monoclonal antibody (Cat # RAB00215) and the cell nuclei were stained with Hoechst 33342 dye (blue).
Immunofluorescent staining of 70% confluent log phase PC-3 cell with CBX3 recombinant monoclonal antibody (Cat # RAB00215). (Panel a: green) under 2 ug/mL working concentration. Nuclei were stained with DAPI (Panel b: blue); F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Panel c: red). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.
Immunoprecipitation analysis of HEK293 cells with CBX3 recombinant monoclonal antibody (Cat # RAB00215). Lane 1: M2 . Lane 2: CBX3 input. Lane 3: CBX3 IP.
Flow cytometric analysis of HT-29 cells with CBX3 recombinant monoclonal antibody (Cat # RAB00215) (red histogram). Yellow histogram: mouse isotype control; Purple histogram: unstained control cells; Green histogram: no primary antibody control.
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. [provided by RefSeq
HP1 gamma homolog,OTTHUMP00000024529,OTTHUMP00000122519,chromobox homolog 3,heterochromatin protein HP1 gamma,heterochromatin-like protein 1