Recombinant rat monoclonal antibody raised against mouse Fcgr3/Fcgr2b.
Original antibody is raised against mouse BALB/c macrophage J774 cell line.
This antibody binds to an epitope on the extracellular domains of the mouse Fc gamma III and Fc gamma II receptors, thereby inhibiting their ability to bind antibodies via their Fc region. Also binding the Fc gamma I receptor (CD64) via its Fc domain.
Protein A affinity purification
Blocking Flow Cytometry (1 ug/mL) Immunofluorescence Immunohistochemistry (5 ug/mL) Immunoprecipitation (10 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (0.02% Proclin 300).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.