His tag (C-term) recombinant monoclonal antibody, clone 3D5
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Specification
Product Description
Rat recombinant monoclonal antibody raised against His tag (C-term).
Antibody Species
Rat
Immunogen
Original antibody is raised against purified His tagged Fab fragment.
Specificity
This antibody recognizes carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA (0.1 ug/mL)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.02% Proclin 300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
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