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Last updated: 2021/4/11
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ERBB2 recombinant monoclonal antibody, clone 4D5-8 (trastuzumab) 

  • Catalog # : RAB00109
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  • Specification
  • Product Description:
  • Recombinant rabbit monoclonal antibody raised against human ERBB2.
  • Immunogen:
  • Original antibody is raised against A431 cells (human epidermoid carcinoma) (over)expressing EGFR.
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A affinity purification
  • Isotype:
  • IgG, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunohistochemistry
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (0.02% Proclin 300).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
    This antibody binds to erbB-2/Her-2/neu has therapeutic indications particularly in the treatment of breast cancer (c.f. Herceptin). It can also be used to detect HER-2 by flow cytometry or IHC.
  • Antibody Species:
  • Rabbit
  • Applications
  • Immunohistochemistry
  • Immunohistochemistry
  • Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the ERBB2 recombinant monoclonal antibody, clone 4D5-8 (trastuzumab) (Cat # RAB00109). Antigen retreival was achieved by microwaving in citrate buffer (pH 6), followed by blocking with protein block serum-free buffer (Dako, cat # X0909). Primary antibody incubation with Cat # RAB00109 was carried out at 4 ug/mL for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody (Dako cat # K4009) for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/mL.
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 2064
  • Gene Name:
  • ERBB2
  • Gene Alias:
  • CD340,HER-2,HER-2/neu,HER2,NEU,NGL,TKR1
  • Gene Description:
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • Gene Summary:
  • This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq
  • Other Designations:
  • c-erb B2/neu protein,erbB-2,herstatin,neuroblastoma/glioblastoma derived oncogene homolog,v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
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