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Last updated: 2020/8/2

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RNA Prestain Marker High 

  • Catalog # : R0019
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  • Specification
  • Product Description:
  • The RNA Prestain Marker High is a RNA ladder for precise sizing of RNA molecules in denaturing agarose gels. The RNA Prestain Marker High is a visible molecular weight marker for ssRNA, consisting of six colored (blue and purple) nucleic acids. The six colored bands (apparent molecular weights are 200, 500, 1,000, 2,000, 4,000 and 8,000 bases) are suitable for monitoring denaturing agarose gel electrophoresis and blotting onto membranes. The RNA Prestain Marker High shows the same mobility as that of the RNA Marker High (Cat # R0003) on denaturing agarose gel electrophoresis (>90% accuracy, see table 1). The RNA Prestain Marker High is supplied in a ready-to-use mixture without requiring heating or the addition of a denaturing agent before use.
  • Quality Control Testing:
  • After 24 hrs incubation of the RNA Prestain Marker High at 37°C, no visible degradation of the marker is observed in 1% agarose- 2.2 M formaldehyde gel electrophoresis.

    QC Testing of R0019
    Electrophoresis profile of RNA Prestain Marker High (6 uL) on 1% agarose - 2.2 M forumaldehyde gel / 1 x MOPS buffer as running buffer.
  • Recommend Usage:
  • Recommended loading volumes:
    Comb (Load volume)
    4~6 mm (4~6 uL)
    >6 mm (>6 uL)
  • Storage Buffer:
  • In 40 mM MOPS, 10 mM AcONa, pH 7.0 (1 mM EDTA-2Na, 10% glycerol).
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • For accurate electrophoretic determination of molecular weights, the RNA Marker High (Cat # R0003) or RNA Marker High Easy (Cat # R0004) should be used.
    The migration of the RNA Prestain Marker High has been optimized to use 0.8-1.5% of agarose gel concentration (see table 1).
    Particularly avoid freeze-thaw cycle.
    It is not possible to use for acrylamide gel electrophoresis.
  • Table 1: Apparent molecular weights as a percentage compared to the RNA Marker High (Cat # R0003).

  • Applications
  • Electrophoresis
  • Application Image
  • Electrophoresis
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