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Last updated: 2020/8/9

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Small RNA Prestain Marker Plus 

  • Catalog # : R0018
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  • Specification
  • Product Description:
  • The Small RNA Prestain Marker Plus is a RNA ladder for precise sizing of RNA molecules (micro RNA) in denaturing acrylamide gels. miRNA is known as a non-coding small RNA, involved in many biological events. The miRNA is derived from precursor miRNA (pre-miRNA, about 100 nt) which is cleaved by the RNase III enzyme Drosha from pri-miRNA. The Small RNA Prestain Marker Plus consists of six prestained single-strand (blue and red) nucleic acids (apparent molecular weights are 100, 75, 50, 40, 30 and 20 bases) and it is visible during electrophoresis. This marker is suitable for monitoring denaturing polyacrylamide gel electrophoresis and blotting onto membranes. The apparent sizes of bands in this marker are in excellent agreement with sizes of non-stained RNAs, 100, 75, 50, 40, 30 and 20 bases in length (about 95% accuracy). This Small RNA Prestain Marker Plus is supplied in a ready-to-use mixture and doesn't require heating or addition of a denaturing agent before use.
  • Quality Control Testing:
  • After 24-hrs incubation of the Small RNA Prestain Marker Plus at 37°C, no visible degradation of the marker is observed in 10% polyacrylamide - 7.5 M urea gel electrophoresis.

    QC Testing of R0018
    Electrophoresis profile of Small RNA Prestain Marker Plus (5 uL) on 10% polyacrylamide - 7.5 M urea gel / 1 x TBE buffer as running buffer.
  • Recommend Usage:
  • Recommended loading volumes:
    Comb (Load volume)
    4-10 mm (5-10 uL)
    >10 mm (>10 uL)
  • Storage Buffer:
  • In 2 mM Tris-HCl, pH 8.0 (8 mM EDTA, 78% formamide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • For accurate electrophoretic determination of molecular weights, the Small RNA Marker (Cat # R0007) or Small RNA Marker Easy (Cat # R0008) should be used.
    A migration of the Small RNA Prestain Marker Plus is optimized to use 10-15% acrylamide gel electrophoresis (see table 1).
    This product is not for agarose gel electrophoresis.
  • Table 1. This shows apparent molecular weights compared with the Small RNA Marker (Cat # R0007), and suitable acrylamide concentrations for electrophoresis of the Small RNA Prestain Marker Plus (Cat # R0018). (* 75 base RNA is from a newly synthesized RNA. A 75 base RNA is not included in Small RNA Marker (Cat # R0007).)

  • Applications
  • Electrophoresis
  • Application Image
  • Electrophoresis
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