RNA Staining Solution (X 1) is developed to stain RNA in polyacrylamide gel and to excise RNA from the gel. The detection limit of RNA is as low as 50 ng. The sensitivity is approximately five times higher than that of UV shadowing. Stained RNA band excised from polyacrylamide gel can be extracted by a crush and soak method followed by ethanol precipitation. Obtained RNA can be used for RT-PCR, enzyme reaction and labeling reaction. RNA Staining Solution (X 1) does not require a UV transilluminator to detect RNA on polyacrylamide gel because bands are visible under daylight.
Quality Control Testing:
After 18 hrs incubation of mixture of RNA Staining Solution 1X and RNA at room temperature (20° -25°C), no visible degradation of RNA is observed in polyacrylamide-urea gel electrophoresis.
Store at 20-25°C for 6 month from the date of receipt. For long term storage, store at 4°C.
RNA is very sensitive to degradation by nucleases, although nucleases do not so easily reach to RNA in polyacrylamide gel. To avoid contamination of nuclease, wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyr°Carbonate (DEPC). Nuclease-free disposable plasticware should be used. Wear gloves and protecting clothing while handling this kit. The stain of RNA Staining Solution is strong.