The RNA Marker High Easy is manufactured for easy measurement of RNA size on agarose gel electrophoresis. With RNA Marker High Easy, electrophorsis is possible on non-denaturing agarose gel as well as on denaturing agarose gel. To use RNA Marker High Easy, just mix RNA loading buffer AG with formaldehyde solution (not included in this kit), and added the mixture to RNA Marker High Easy or RNA sample, then heat to load into wells of agarose gel. The RNA loading buffer AG+ is a gel loading buffer prepared not only for denaturing agarose gel electrophoresis but also for non-contained denaturing agarose gel electrophoresis. RNA Marker High Easy contains nine single-stranded RNAs, 500, 1,000, 1,500, 2,000, 3,000, 4,000, 5,000 and 8,000 bases. Each RNA is 0.1 ug/ul approximately. It is useful for estimating RNA amount approximately. After electrophoresis, RNA bands on agarose gel can be visualized by UV light without further ethidium bromide staining.
Quality Control Testing:
After 18 hr incubation of RNA Marker High Easy at 37°C, no visible degradation of the marker is observed in formaldehyde-agarose (1%) gel electrophoresis.
RNA loading buffer B
Store at -80 °C. Repeated freeze/thaw cycles should be avoided.
RNA is very sensitive to degradation by nuclease. To prevent nuclease contamination, extreme care is required for manipulation. Wear gloves and use clean apparatus. Glassware should be pretreated with diethyl pyrocarbonate (DEPC). Nuclease-free disposable plasticware should be used. Solutions and reagents to mix the marker should be high grade and nuclease-free. To use, thaw the RNA Marker High Easy on ice. Keep RNA Marker High Easy and RNA loading buffer B on ice while using. This kit can be used for non-denaturing agarose gel electrophoresis. Although adequate results to size RNAs were obtained as far as we tested, denaturing agarose gel electrophoresis may be required for a strict experiment. Formamide is suspected to be harmful. It is irritate to the eyes and skin. Ethidium bromide is a strong mutagen and susupected to be toxic. Wear appropriate gloves and safety glasses in using solutions and materials containing formamide and/or etidium bromide. Put a lid tightly at the time of storage. Formaldehyde is carcinogen and toxic. Avoid breathing the vapors. Wear appropriate gloves and safety glasses. Gel containing formaldehyde should be prepared and used in a chemical hood.