TDGF1 polyclonal antibody
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More Files
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Western Blot
Western blot using TDGF1 polyclonal antibody (Cat # PAB9995) shows detection of endogenous and transfected TDGF1 from mouse and human sources.
The TDGF1 band appears above the 17.8 KDa marker.
Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells.
EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse TDGF1.
NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose TDGF1 expression.
COS-7 cells transfected with human TDGF1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control.
A non-specific band at 45 KDa may be present in some preparations.
The primary antibody was used at a 1 : 500 dilution.
Personal communication, C. Bianco, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TDGF1.
Immunogen
A synthetic peptide corresponding to internal region of human TDGF1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:105000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using TDGF1 polyclonal antibody (Cat # PAB9995) shows detection of endogenous and transfected TDGF1 from mouse and human sources.
The TDGF1 band appears above the 17.8 KDa marker.
Endogenous detection is shown using mouse P19 embryonal carcinoma cells and human NCCIT testicular embryonal carcinoma cells.
EpH4 CR-1 is a mouse mammary epithelial cell line stably expressing mouse TDGF1.
NTERA cells are human embryonal carcinoma cells that, when overgrown, differentiate and lose TDGF1 expression.
COS-7 cells transfected with human TDGF1 expression vector were used as a positive control and EpH4 WT cells were used as a negative control.
A non-specific band at 45 KDa may be present in some preparations.
The primary antibody was used at a 1 : 500 dilution.
Personal communication, C. Bianco, NCI, Bethesda, MD.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — TDGF1
Entrez GeneID
6997Protein Accession#
NP_003203;P13385Gene Name
TDGF1
Gene Alias
CR, CRGF, CRIPTO, Cripto-1
Gene Description
teratocarcinoma-derived growth factor 1
Omim ID
187395Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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Publication Reference
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Role of human cripto-1 in tumor angiogenesis.
Bianco C, Strizzi L, Ebert A, Chang C, Rehman A, Normanno N, Guedez L, Salloum R, Ginsburg E, Sun Y, Khan N, Hirota M, Wallace-Jones B, Wechselberger C, Vonderhaar BK, Tosato G, Stetler-Stevenson WG, Sanicola M, Salomon DS.
Journal of the National Cancer Institute 2005 Jan; 97(2):132.
Application：Func, Human, HUVECs, MCF-7 cells.
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Cripto-1: an oncofetal gene with many faces.
Bianco C, Strizzi L, Normanno N, Khan N, Salomon DS.
Current Topics in Developmental Biology 2005 Jan; 67:85.
Application：IHC, WB, Human, Human cancers.
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Role of human cripto-1 in tumor angiogenesis.
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