CDK5 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CDK5 polyclonal antibody (Cat # PAB9990) was diluted 1 : 500 to detect CDK5 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDK5.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CDK5.
Host
Rabbit
Theoretical MW (kDa)
35
Reactivity
Human, Mouse, Rat
Specificity
Antiserum will specifically react with a 35 KDa cdk5 (p31) protein from human, rat and mouse tissue. Cross reactivity with cdk5 (p31) from other species may also occur.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:500-1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CDK5 polyclonal antibody (Cat # PAB9990) was diluted 1 : 500 to detect CDK5 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDK5
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Interactome
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Pathway
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Disease
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Publication Reference
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Neuronal cdc2-like kinase: a cdc2-related protein kinase with predominantly neuronal expression.
Hellmich MR, Pant HC, Wada E, Battey JF.
PNAS 1992 Nov; 89(22):10867.
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A family of human cdc2-related protein kinases.
Meyerson M, Enders GH, Wu CL, Su LK, Gorka C, Nelson C, Harlow E, Tsai LH.
The EMBO Journal 1992 Aug; 11(8):2909.
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Neuronal cdc2-like kinase: a cdc2-related protein kinase with predominantly neuronal expression.
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