Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC27.
Synthetic phosphopeptide corresponding to residues surrounding S427 of human CDC27.
Chicken, Chimpanzee, Dog, Human, Mouse, Rat
Reactivity occurs against human CDC27 pS427 protein and This antibody is specific to the phosphorylated form of the protein. This antibody does not cross-react with CDC27 phosphorylated at other sites.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:5000-1:25000 Western Blot (1:500-1:2500) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
anaphase-promoting complex, protein 3,cell division cycle protein 27,nuc2 homolog