CDC27 (phospho S427) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC27.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S427 of human CDC27.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Dog, Human, Mouse, Rat
Specificity
Reactivity occurs against human CDC27 pS427 protein and This antibody is specific to the phosphorylated form of the protein. This antibody does not cross-react with CDC27 phosphorylated at other sites.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:25000
Western Blot (1:500-1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC27
Entrez GeneID
996Protein Accession#
P30260;NP_001247Gene Name
CDC27
Gene Alias
APC3, CDC27Hs, D0S1430E, D17S978E, HNUC
Gene Description
cell division cycle 27 homolog (S. cerevisiae)
Omim ID
116946Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
anaphase-promoting complex, protein 3|cell division cycle protein 27|nuc2 homolog
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Interactome
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Pathway
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Publication Reference
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Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression.
Gabellini D, Colaluca IN, Vodermaier HC, Biamonti G, Giacca M, Falaschi A, Riva S, Peverali FA.
The EMBO Journal 2003 Jul; 22(14):3715.
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The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis.
Topper LM, Campbell MS, Tugendreich S, Daum JR, Burke DJ, Hieter P, Gorbsky GJ.
Cell Cycle 2002 Jul; 1(4):282.
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Mad2 transiently associates with an APC/p55Cdc complex during mitosis.
Wassmann K, Benezra R.
PNAS 1998 Sep; 95(19):11193.
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Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression.
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