CBLC polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry CBLC polyclonal antibody (Cat # PAB9985) shows strong nuclear and cytoplasmic staining of cells in tubuli in human kidney tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of CBLC polyclonal antibody (Cat # PAB9985) was used at 5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate intracellular positive staining of human pancreatic acinar epithelium at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
Immunoprecipitation
Immunoprecipitation and western blot using CBLC polyclonal antibody (Cat # PAB9985) shows detection of a pre-dominant band at ~52 kDa corresponding to CBLC (arrowhead) in transfected cell lysates (left panel).
Lysates are from HEK 293T cells transfected with empty vector or with CBLC.
The predicted size of CBLC is 52 kDa.
Size markers in kDa are shown to the left of the panel.
The right panel shows western blotting after first immunoprecipitating with Rabbit anti-CBLC followed by immunoblotting using a Goat anti-CBLC antibody.
Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CBLC.
Immunogen
A synthetic peptide corresponding to amino acids 444-458 of human CBLC.
Host
Rabbit
Reactivity
Chimpanzee, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry CBLC polyclonal antibody (Cat # PAB9985) shows strong nuclear and cytoplasmic staining of cells in tubuli in human kidney tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of CBLC polyclonal antibody (Cat # PAB9985) was used at 5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate intracellular positive staining of human pancreatic acinar epithelium at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.Immunoprecipitation
Immunoprecipitation and western blot using CBLC polyclonal antibody (Cat # PAB9985) shows detection of a pre-dominant band at ~52 kDa corresponding to CBLC (arrowhead) in transfected cell lysates (left panel).
Lysates are from HEK 293T cells transfected with empty vector or with CBLC.
The predicted size of CBLC is 52 kDa.
Size markers in kDa are shown to the left of the panel.
The right panel shows western blotting after first immunoprecipitating with Rabbit anti-CBLC followed by immunoblotting using a Goat anti-CBLC antibody.
Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CBLC
Entrez GeneID
23624Protein Accession#
Q9ULV8Gene Name
CBLC
Gene Alias
CBL-3, CBL-SL, RNF57
Gene Description
Cas-Br-M (murine) ecotropic retroviral transforming sequence c
Omim ID
608453Gene Ontology
HyperlinkGene Summary
CBL proteins, such as CBLC, are phosphorylated upon activation of a variety of receptors that signal via protein tyrosine kinases. Through interactions with proteins containing SRC (MIM 190090) homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling (Keane et al., 1999 [PubMed 10362357]).[supplied by OMIM
Other Designations
Cas-Br-M (murine) ectropic retroviral transforming sequence c
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Interactome
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Pathway
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Publication Reference
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Molecular cloning and characterization of a novel cbl-family gene, cbl-c.
Kim M, Tezuka T, Suziki Y, Sugano S, Hirai M, Yamamoto T.
Gene 1999 Oct; 239(1):145.
Application：IP, WB-Ce, WB-Tr, Human, COLO320DM, DLD-1, HCT-15, HEK 293T cells.
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cbl-3: a new mammalian cbl family protein.
Keane MM, Ettenberg SA, Nau MM, Banerjee P, Cuello M, Penninger J, Lipkowitz S.
Oncogene 1999 Jun; 18(22):3365.
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Molecular cloning and characterization of a novel cbl-family gene, cbl-c.
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