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Last updated: 2016/10/16
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CAMK2G polyclonal antibody

  • Catalog # : PAB9984
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CAMK2G.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 6-23 of human CAMK2G.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Dog, Frog, Human, Mouse, Orangutan, Pig, Rabbit, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000-1:20000)
    Western Blot (1:1000-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using CAMK2G polyclonal antibody (Cat # PAB9984) shows detectionof a band ~54 KDa corresponding to human CAMK2G (arrowhead lane 1).
    Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (Lane 2).
    Approximately 35 ug of a mouse brain tissue lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
    CAMK2G was similarly detected on lysates from rat brain (not shown).
    After blocking the membrane was probed with the primary antibody diluted to 1 : 1,500 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademarkof LI-COR, Inc.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 818
  • Protein Accession#:
  • Q9UQM7(Human);P11798(Mouse);P11275(Rat)
  • Gene Name:
  • CAMK2G
  • Gene Alias:
  • Gene Description:
  • calcium/calmodulin-dependent protein kinase II gamma
  • Gene Summary:
  • The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Six alternatively spliced variants that encode six different isoforms have been characterized to date. Additional alternative splice variants that encode different isoforms have been described, but their full-length nature has not been determined. [provided by RefSeq
  • Other Designations:
  • CaM kinase II,OTTHUMP00000019843,OTTHUMP00000019844,calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma
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