Western blot using CTCFL polyclonal antibody (Cat # PAB9983) shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead). Lane 1 contains lysate from human prostate tissue. Lane 2 contains lysate from human spleen tissue. Apredominant band at ~75 KDa is observed. Molecular weight estimation was made by comparison to prestained MW markers as indicated.
CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. [provided by RefSeq
BORIS-like protein,CCCTC-binding factor-like protein,OTTHUMP00000174287,brother of the regulator of imprinted sites