BMI1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using BMI1 polyclonal antibody (Cat # PAB9982) shows detection of a band ~37 KDa corresponding to human BMI1 (arrowhead).
Approximately 20 ug of a U-2 OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4°C with the primary antibody diluted to 1 : 1,000 in PBS containing 1% nonfat dry milk.
The membrane was washed and reacted with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of BMI1.
Immunogen
A synthetic peptide corresponding to amino acids 252-264 of human BMI1.
Host
Goat
Reactivity
Bovine, Cat, Chicken, Chimpanzee, Clawed frog, Dog, Human, Orangutan, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:30000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using BMI1 polyclonal antibody (Cat # PAB9982) shows detection of a band ~37 KDa corresponding to human BMI1 (arrowhead).
Approximately 20 ug of a U-2 OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4°C with the primary antibody diluted to 1 : 1,000 in PBS containing 1% nonfat dry milk.
The membrane was washed and reacted with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — BMI1
Entrez GeneID
648Protein Accession#
NP_005171;P35226Gene Name
BMI1
Gene Alias
MGC12685, PCGF4, RNF51
Gene Description
BMI1 polycomb ring finger oncogene
Omim ID
164831Gene Ontology
HyperlinkOther Designations
B lymphoma Mo-MLV insertion region 1 homolog|flvi-2/bmi-1|murine leukemia viral (bmi-1) oncogene homolog|oncogene BMI-1|polycomb group ring finger 4
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Interactome
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Publication Reference
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MAPKAP kinase 3pK phosphorylates and regulates chromatin association of the polycomb group protein Bmi1.
Voncken JW, Niessen H, Neufeld B, Rennefahrt U, Dahlmans V, Kubben N, Holzer B, Ludwig S, Rapp UR.
The Journal of Biological Chemistry 2005 Feb; 280(7):5178.
Application：IF, WB-Ce, Human, HeLa, 293T, TIG3, U2OS cells.
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Bmi1 is essential for cerebellar development and is overexpressed in human medulloblastomas.
Leung C, Lingbeek M, Shakhova O, Liu J, Tanger E, Saremaslani P, Van Lohuizen M, Marino S.
Nature 2004 Mar; 428(6980):337.
Application：IHC, WB, Human, Mouse , CGCs, D-341, D-458, DAOY, RK3E cells, Human medulloblastomas.
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Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase.
Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K.
Genes to Cells 2004 Feb; 9(2):105.
Application：IF, WB-Ce, Human, HeLa, MRC5 cells.
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MAPKAP kinase 3pK phosphorylates and regulates chromatin association of the polycomb group protein Bmi1.
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