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Last updated: 2016/12/4
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BMI1 polyclonal antibody

  • Catalog # : PAB9982
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of BMI1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 252-264 of human BMI1.
  • Host:
  • Goat
  • Reactivity:
  • Bovine, Cat, Chicken, Chimpanzee, Clawed frog, Dog, Human, Orangutan, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:30000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using BMI1 polyclonal antibody (Cat # PAB9982) shows detection of a band ~37 KDa corresponding to human BMI1 (arrowhead).
    Approximately 20 ug of a U-2 OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
    After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4°C with the primary antibody diluted to 1 : 1,000 in PBS containing 1% nonfat dry milk.
    The membrane was washed and reacted with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 648
  • Protein Accession#:
  • NP_005171;P35226
  • Gene Name:
  • BMI1
  • Gene Alias:
  • MGC12685,PCGF4,RNF51
  • Gene Description:
  • BMI1 polycomb ring finger oncogene
  • Other Designations:
  • B lymphoma Mo-MLV insertion region 1 homolog,flvi-2/bmi-1,murine leukemia viral (bmi-1) oncogene homolog,oncogene BMI-1,polycomb group ring finger 4
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