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Last updated: 2016/12/4
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TRIM29 polyclonal antibody

  • Catalog # : PAB9977
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant TRIM29.
  • Immunogen:
  • Recombinant protein corresponding to internal region around acetylated K116 of human TRIM29.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chimpanzee, Horse, Human, Macaque, Mouse, Rat
  • Specificity:
  • This antibody reacts with over-expressed, acetylated lysine ATDC protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:100000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using TRIM29 polyclonal antibody (Cat # PAB9977) shows detection of a 66 kDa band corresponding to over-expressed, acetylated lysine (K116) TRIM29 (arrowhead) in transfected 293T cells.
    No staining is noted for cells transfected with empty vector only.
    No staining is noted for cells transfected with an TRIM29 mutant (K to R transversion lacks site for acetylation).
    In each instance, samples were prepared with and without TSA (1.3 uM, 6 hr) which inhibits deacetylation.
    Personal communication, Z. Yuan, H Lee Moffitt Cancer Center and Research Institute.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_036233;Q14134
  • Gene Name:
  • TRIM29
  • Gene Alias:
  • ATDC,FLJ36085
  • Gene Description:
  • tripartite motif-containing 29
  • Gene Summary:
  • The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. [provided by RefSeq
  • Other Designations:
  • ataxia-telangiectasia group D-associated protein,tripartite motif protein TRIM29
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