TRIM29 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant TRIM29.
Immunogen
Recombinant protein corresponding to internal region around K116 of human TRIM29.
Host
Rabbit
Reactivity
Bovine, Chimpanzee, Horse, Human, Macaque, Mouse, Rat
Specificity
This antibody reacts with over-expressed, acetylated and non-acetylated (at K116) ATDC protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:100000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using TRIM29 polyclonal antibody (Cat # PAB9976) shows reactivity by dot blot with acetylated and non-acetylated forms of the immunizing peptide.
This antibody is predicted to recognize both acetylated (TRIM29) and non-acetylated forms of TRIM29 protein.
Personal communication, Z. Yuan, H Lee Moffitt Cancer Center and Research Institute.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRIM29
Entrez GeneID
23650Protein Accession#
NP_036233;Q14134Gene Name
TRIM29
Gene Alias
ATDC, FLJ36085
Gene Description
tripartite motif-containing 29
Omim ID
610658Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. [provided by RefSeq
Other Designations
ataxia-telangiectasia group D-associated protein|tripartite motif protein TRIM29
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Interactome
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Disease
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Publication Reference
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The product of the ataxia-telangiectasia group D complementing gene, ATDC, interacts with a protein kinase C substrate and inhibitor.
Brzoska PM, Chen H, Zhu Y, Levin NA, Disatnik MH, Mochly-Rosen D, Murnane JP, Christman MF.
PNAS 1995 Aug; 92(17):7824.
Application:IF, Human, LM217 cells.
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Nucleotide sequence analysis of a candidate gene for ataxia-telangiectasia group D (ATDC).
Leonhardt EA, Kapp LN, Young BR, Murnane JP.
Genomics 1994 Jan; 19(1):130.
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Cloning of a candidate gene for ataxia-telangiectasia group D.
Kapp LN, Painter RB, Yu LC, van Loon N, Richard CW 3rd, James MR, Cox DR, Murnane JP.
American Journal of Human Genetics 1992 Jul; 51(1):45.
Application:WB-Ce, WB-Tr, Human, HeLa cells, Mammalian cells.
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The product of the ataxia-telangiectasia group D complementing gene, ATDC, interacts with a protein kinase C substrate and inhibitor.
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