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Last updated: 2017/12/17
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PPP1R13B polyclonal antibody

  • Catalog # : PAB9974
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PPP1R13B.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human PPP1R13B.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2500-1:10000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using PPP1R13B polyclonal antibody (Cat # PAB9974) to detect over-expressed PPP1R13B in MCF-7 cells (Lane 2, arrowhead).
    Lane 1 is a non-transfected control.
    Lane 3 is MCF-7 cells over-expressing TP53BP2.
    Cell extracts were electrophoresed and transferred to nitrocellulose.
    The membrane was probed with the primary antibody at a 1 : 1,000 dilution.
    The identity of the lower MW band at approximately 50 KDa is unknown.
    Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
    Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_056131;Q96KQ4
  • Gene Name:
  • PPP1R13B
  • Gene Alias:
  • ASPP1,KIAA0771,p53BP2-like,p85
  • Gene Description:
  • protein phosphatase 1, regulatory (inhibitor) subunit 13B
  • Gene Summary:
  • This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor. [provided by RefSeq
  • Other Designations:
  • apoptosis-stimulating protein of p53, 1
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