PPP1R13B polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PPP1R13B.
Immunogen
A synthetic peptide corresponding to internal region of human PPP1R13B.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2500-1:10000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using PPP1R13B polyclonal antibody (Cat # PAB9974) to detect over-expressed PPP1R13B in MCF-7 cells (Lane 2, arrowhead).
Lane 1 is a non-transfected control.
Lane 3 is MCF-7 cells over-expressing TP53BP2.
Cell extracts were electrophoresed and transferred to nitrocellulose.
The membrane was probed with the primary antibody at a 1 : 1,000 dilution.
The identity of the lower MW band at approximately 50 KDa is unknown.
Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP1R13B
Entrez GeneID
23368Protein Accession#
NP_056131;Q96KQ4Gene Name
PPP1R13B
Gene Alias
ASPP1, KIAA0771, p53BP2-like, p85
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 13B
Omim ID
606455Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor. [provided by RefSeq
Other Designations
apoptosis-stimulating protein of p53, 1
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Interactome
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Disease
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Publication Reference
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ASPP [corrected] and cancer.
Trigiante G, Lu X.
Nature Reviews. Cancer 2006 Mar; 6(3):217.
Application:IP, WB-Tr, Human, HEK 293 cells.
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ASPP--Apoptotic specific regulator of p53.
Liu ZJ, Lu X, Zhong S.
Biochimica et Biophysica Acta 2005 Sep; 1756(1):77.
Application:WB, Human, Human mammalian cells .
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The ASPP family: deciding between life and death after DNA damage.
Slee EA, Lu X.
Toxicology Letters 2003 Apr; 139(2-3):81.
Application:WB-Tr, Human, Mammalian cells.
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ASPP [corrected] and cancer.
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