AP3D1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot using AP3D1 polyclonal antibody (Cat # PAB9965) shows detection of a 130 KDa band corresponding to Human AP3D1 in a HeLa whole cell lysate.
The lower molecular weight band most likely represents non-specificbinding.
Approximately 20 ug of lysate was run ona SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP3D1 polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-GoatIgG for 1 hour at room temperature.
A chemilumi-nescence system was used for signal detection using a 30-sec exposure time.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AP3D1.
Immunogen
A synthetic peptide corresponding to amino acids 686-698 of human AP3D1.
Host
Goat
Reactivity
Bovine, Dog, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot using AP3D1 polyclonal antibody (Cat # PAB9965) shows detection of a 130 KDa band corresponding to Human AP3D1 in a HeLa whole cell lysate.
The lower molecular weight band most likely represents non-specificbinding.
Approximately 20 ug of lysate was run ona SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP3D1 polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-GoatIgG for 1 hour at room temperature.
A chemilumi-nescence system was used for signal detection using a 30-sec exposure time.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — AP3D1
Entrez GeneID
8943Protein Accession#
O14617;NP_003929Gene Name
AP3D1
Gene Alias
ADTD, hBLVR
Gene Description
adaptor-related protein complex 3, delta 1 subunit
Omim ID
607246Gene Ontology
HyperlinkGene Summary
AP3D1 is a subunit of the AP3 adaptor-like complex, which is not associated with clathrin. The AP3D1 subunit is implicated in intracellular biogenesis and trafficking of pigment granules and possibly platelet dense granules and neurotransmitter vesicles.[supplied by OMIM
Other Designations
AP-3 complex delta subunit, partial CDS|adaptin, delta|delta adaptin|delta-adaptin, partial CDS|subunit of putative vesicle coat adaptor complex AP-3
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Altered expression of a novel adaptin leads to defective pigment granule biogenesis in the Drosophila eye color mutant garnet.
Ooi CE, Moreira JE, Dell'Angelica EC, Poy G, Wassarman DA, Bonifacino JS.
The EMBO Journal 1997 Aug; 16(15):4508.
Application：IP, WB-Ce, Human, Jurkat cells.
-
Characterization of the adaptor-related protein complex, AP-3.
Simpson F, Peden AA, Christopoulou L, Robinson MS.
The Journal of Cell Biology 1997 May; 137(4):835.
Application：IF, IP, WB-Ti, Pig, MDBK, NRK cells, Pig brains.
-
Altered expression of a novel adaptin leads to defective pigment granule biogenesis in the Drosophila eye color mutant garnet.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com