ABCB1 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using ABCB1 polyclonal antibody (Cat # PAB9962) shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1.
The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose.
The membrane was probed with the primary antibody diluted to 1 : 600, followed by Peroxidase Conjugated Affinity Purified Anti-Rabbit IgG at 1 : 10,000.
Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ABCB1.
Immunogen
A synthetic peptide corresponding to amino acids 262-277 of human ABCB1.
Host
Rabbit
Reactivity
Dog, Human, Monkey, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using ABCB1 polyclonal antibody (Cat # PAB9962) shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1.
The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose.
The membrane was probed with the primary antibody diluted to 1 : 600, followed by Peroxidase Conjugated Affinity Purified Anti-Rabbit IgG at 1 : 10,000.
Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ABCB1
Entrez GeneID
5243Protein Accession#
NP_000918;P08183Gene Name
ABCB1
Gene Alias
ABC20, CD243, CLCS, GP170, MDR1, MGC163296, P-GP, PGY1
Gene Description
ATP-binding cassette, sub-family B (MDR/TAP), member 1
Omim ID
171050Gene Ontology
HyperlinkGene Summary
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq
Other Designations
ATP-binding cassette, subfamily B, member 1|P glycoprotein 1|P-glycoprotein 1|colchicin sensitivity|doxorubicin resistance|multidrug resistance 1|multidrug resistance protein 1
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Publication Reference
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The remarkable transport mechanism of P-glycoprotein: a multidrug transporter.
Al-Shawi MK, Omote H.
Journal of Bioenergetics and Biomembranes 2005 Dec; 37(6):489.
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Epigenetic changes to the MDR1 locus in response to chemotherapeutic drugs.
Baker EK, Johnstone RW, Zalcberg JR, El-Osta A.
Oncogene 2005 Dec; 24(54):8061.
Application：WB, Human, CEM-Bcl2, SW620 cells.
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Modulation of MDR1 gene expression in multidrug resistant MCF7 cells by low concentrations of small interfering RNAs.
Stierlé V, Laigle A, Jollès B.
Biochemical Pharmacology 2005 Nov; 70(10):1424.
Application：WB, Human, MCF-7 cells.
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The remarkable transport mechanism of P-glycoprotein: a multidrug transporter.
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