Rabbit polyclonal antibody raised against synthetic phosphopeptide of MYL6.
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S19/S20 of human MYL6.
This antibody is phosphospecific and detect monophosphorylated and diphosphorylated forms of the protein. This phosphospecific polyclonal antibody is specific to the phosphorylated pS19/pS20 form of the protein, depending on the source origin of the protein.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:120000-1:135000) Western Blot (1:1000-1:5000) Immunohistochemistry (2.5 ug/mL) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at a 1 : 5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 uL of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system. Other detection methods will yield similar results. Data courtesy of the Alliance for Cellular Signaling (http://www.signaling-gateway.org).
Western Blot (Cell lysate)
MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at a 1 : 1000 dilution to detect myosin light chain by Western blot on NIH/3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown.The phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain. Personal communication. J. Stull. UT Southwestern Medical Center.
Immunohistochemical staining with MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at 2.5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
myosin, light polypeptide 6, alkali, smooth muscle and non-muscle,smooth muscle and non-muscle myosin alkali light chain