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Last updated: 2017/8/13
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MYL6 (phospho S19/20) polyclonal antibody

  • Catalog # : PAB9961
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of MYL6.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S19/S20 of human MYL6.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is phosphospecific and detect monophosphorylated and diphosphorylated forms of the protein. This phosphospecific polyclonal antibody is specific to the phosphorylated pS19/pS20 form of the protein, depending on the source origin of the protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:120000-1:135000)
    Western Blot (1:1000-1:5000)
    Immunohistochemistry (2.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at a 1 : 5000 dilution to detect myosin light chain by Western blot.
    Either 13 or 20 uL of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
    Other detection methods will yield similar results.
    Data courtesy of the Alliance for Cellular Signaling (http://www.signaling-gateway.org).
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at a 1 : 1000 dilution to detect myosin light chain by Western blot on NIH/3T3 cell lysates.
    A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios.
    In Panel A, reactivity of MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) is shown.
    In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown.The phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain.
    Personal communication. J. Stull. UT Southwestern Medical Center.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining with MYL6 (phospho S19/20) polyclonal antibody (Cat # PAB9961) was used at 2.5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus.
    Tissue was formalin-fixed and paraffin embedded.
    The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4637
  • Gene Name:
  • MYL6
  • Gene Alias:
  • ESMLC,LC17-GI,LC17-NM,LC17A,LC17B,MLC1SM,MLC3NM,MLC3SM
  • Gene Description:
  • myosin, light chain 6, alkali, smooth muscle and non-muscle
  • Gene Summary:
  • Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • myosin, light polypeptide 6, alkali, smooth muscle and non-muscle,smooth muscle and non-muscle myosin alkali light chain
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