MAPK1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using MAPK1 polyclonal antibody (Cat # PAB9958) shows detection of MAPK1 in several whole cell lysates : HeLa (lane 1), A-431 (lane 2), and NIH/3T3 (lane 3).
Detection occurs using a 1 : 1,000 dilution of the primary antibody followed by 1 : 4,000 dilution of HRP Goat-a-Rabbit with visualization via ECL.
Film exposure was approximately 1'.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant MAPK1.
Immunogen
Recombinant GST fusion protein corresponding to C-terminus of human MAPK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:1000-1:2000)
Immunoprecipitation (5.0 ug/mg)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using MAPK1 polyclonal antibody (Cat # PAB9958) shows detection of MAPK1 in several whole cell lysates : HeLa (lane 1), A-431 (lane 2), and NIH/3T3 (lane 3).
Detection occurs using a 1 : 1,000 dilution of the primary antibody followed by 1 : 4,000 dilution of HRP Goat-a-Rabbit with visualization via ECL.
Film exposure was approximately 1'.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK1
Entrez GeneID
5594Protein Accession#
P28482;NP_002736Gene Name
MAPK1
Gene Alias
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
Gene Description
mitogen-activated protein kinase 1
Omim ID
176948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174492|extracellular signal-regulated kinase 2|extracellular signal-regulated kinase-2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
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Interactome
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Pathway
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Disease
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Publication Reference
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The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis.
Asthagiri AR, Reinhart CA, Horwitz AF, Lauffenburger DA.
Journal of cell Science 2000 Dec; 113 Pt 24:4499.
Application：KA, Mouse, CHO cells.
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Organization and regulation of mitogen-activated protein kinase signaling pathways.
Garrington TP, Johnson GL.
Current Opinion in Cell Biology 1999 Apr; 11(2):211.
Application：IHC, WB-Ce, WB-Tr, Human, Cancers, Mammalian cells.
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The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579.
Hoffmann R, Baillie GS, MacKenzie SJ, Yarwood SJ, Houslay MD.
The EMBO Journal 1999 Feb; 18(4):893.
Application：WB-Ce, Monkey, COS-1 cells.
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The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis.
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