Western blot using MAPK1 polyclonal antibody (Cat # PAB9958) shows detection of MAPK1 in several whole cell lysates : HeLa (lane 1), A-431 (lane 2), and NIH/3T3 (lane 3). Detection occurs using a 1 : 1,000 dilution of the primary antibody followed by 1 : 4,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure was approximately 1'.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq