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Western Blot

Immunoprecipitation

Combined immunoprecipitation and immunoblot of a human cell lysate using ERBB3 polyclonal antibody (Cat # PAB9951).
Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-beta1 for 15' at 100 ng/mL and without (-) the addition of Heregulin-beta1.
The combination of immunoprecipitation and immunoblotting was performing using 5 uL of the ERBB3 polyclonal antibody for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody.
Visualization occurred using an ECL system.
Film exposure approximately 1'.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

2065

Gene Name

ERBB3

Gene Alias

ErbB-3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3

Gene Description

v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Omim ID

190151 607598

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq

Other Designations

erbB-3|lethal congenital contracture syndrome 2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3