PTGS2 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
PTGS2 polyclonal antibody (Cat # PAB9950) is shownto detect PTGS2 present in PTGS2 transfected Sf9 cellextract (lane 1) and IL1B induced WISH cell extract (lane 2).
Detection occursusing a 1 : 10,000 dilution of antibody followed by 1 : 10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately1'.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with PTGS2 polyclonal antibody (Cat # PAB9950) was diluted 1 : 500 to detect PTGS2 in human brain cortex tissue.
Tissue was formalin fixed and paraffin embedded.
No pre-treatment of sample was required.
The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant PTGS2.
Immunogen
Recombinant GST fusion protein corresponding to C-terminus of human PTGS2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:5000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
PTGS2 polyclonal antibody (Cat # PAB9950) is shownto detect PTGS2 present in PTGS2 transfected Sf9 cellextract (lane 1) and IL1B induced WISH cell extract (lane 2).
Detection occursusing a 1 : 10,000 dilution of antibody followed by 1 : 10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately1'.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with PTGS2 polyclonal antibody (Cat # PAB9950) was diluted 1 : 500 to detect PTGS2 in human brain cortex tissue.
Tissue was formalin fixed and paraffin embedded.
No pre-treatment of sample was required.
The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTGS2
Entrez GeneID
5743Gene Name
PTGS2
Gene Alias
COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Gene Description
prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Omim ID
600262Gene Ontology
HyperlinkGene Summary
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000033524|cyclooxygenase 2b|prostaglandin G/H synthase and cyclooxygenase|prostaglandin-endoperoxide synthase 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Induction of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase expression by androgens in human prostate cancer cells.
Tong M, Tai HH.
Biochemical and Biophysical Research Communications 2000 Sep; 276(1):77.
Application：WB-Ce, Human, LNCaP cells.
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Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins.
Hittelman AB, Burakov D, Iniguez-Lluhi JA, Freedman LP, Garabedian MJ.
The EMBO Journal 1999 Oct; 18(19):5380.
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Interleukin-1beta-induced cyclooxygenase-2 expression requires activation of both c-Jun NH2-terminal kinase and p38 MAPK signal pathways in rat renal mesangial cells.
Guan Z, Buckman SY, Miller BW, Springer LD, Morrison AR.
The Journal of Biological Chemistry 1998 Oct; 273(44):28670.
Application：WB-Tr, Rat, Mesangial cells.
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Induction of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase expression by androgens in human prostate cancer cells.
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