BIVM polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of BIVM polyclonal antibody (Cat # PAB9949).
Whole cell HeLa lysate was used to probe for endogenous BIVM using Rabbit-anti-Human BIVM (C-terminal specific) polyclonal antibody.
A 57 kDa band corresponding to human BIVM protein is detected using a 1 : 1,000 dilution of the antiserum incubated for 1 hour at room temperature.
Washes with 1% Tween-20 TBS preceded reaction with HRP Gt-a-Rabbit IgG (H&L) diluted 1 : 10,000 and incubated for 1 hour at room temperature.
The blot was developed using a chemiluminescent detection method (AP ECL 60 sec followed by a 45 sec exposure).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BIVM.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human BIVM.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:25000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of BIVM polyclonal antibody (Cat # PAB9949).
Whole cell HeLa lysate was used to probe for endogenous BIVM using Rabbit-anti-Human BIVM (C-terminal specific) polyclonal antibody.
A 57 kDa band corresponding to human BIVM protein is detected using a 1 : 1,000 dilution of the antiserum incubated for 1 hour at room temperature.
Washes with 1% Tween-20 TBS preceded reaction with HRP Gt-a-Rabbit IgG (H&L) diluted 1 : 10,000 and incubated for 1 hour at room temperature.
The blot was developed using a chemiluminescent detection method (AP ECL 60 sec followed by a 45 sec exposure).Enzyme-linked Immunoabsorbent Assay
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Gene Info — BIVM
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Interactome
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Disease
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Publication Reference
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BIVM, a novel gene widely distributed among deuterostomes, shares a core sequence with an unusual gene in Giardia lamblia.
Yoder JA, Hawke NA, Eason DD, Mueller MG, Davids BJ, Gillin FD, Litman GW.
Genomics 2002 Jun; 79(6):750.
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BIVM, a novel gene widely distributed among deuterostomes, shares a core sequence with an unusual gene in Giardia lamblia.
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