Immunoblot of MAP3K5 (phospho S83) polyclonal antibody (Cat # PAB9947) shows specificity for phosphorylated human MAP3K5. MAP3K5 (phospho S83) polyclonal antibody was tested by immunoblot against COS-7 cell lysates after transient transfection with (Lane 1) vector only, (Lane 2) recombinant HA-MAP3K5, and (Lane 3) recombinant human HA-MA3K5 where S83 was substituted with an alanine residue. A : anti-HA. B : MAP3K5 (phospho S83) polyclonal antibody blocked with non-phospho peptide. C : MAP3K5 (phospho S83) polyclonal antibody blocked with phospho peptide. A 155 kDa band corresponding to human MAP3K5 is detected.
ELISA results of MAP3K5 (phospho S83) polyclonal antibody (Cat # PAB9947) tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide. Each well was coated with 0.1 mg of conjugate. The starting dilution of antibody was 1 : 1,000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Goat-anti-Rabbit IgG H&L and TMB substrate were used for detection.
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq
MAP/ERK kinase kinase 5,MAPK/ERK kinase kinase 5,OTTHUMP00000017275,apoptosis signal regulating kinase