Western blot using HIF1A polyclonal antibody, P564 (Cat # PAB9946) shows detection (left panel) of hydroxylated HIF1A in nuclear extracts of A-549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF1A when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional HIF1A polyclonal antibody. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1A. The primary antibody was used at a 1 : 1,000 dilution in 2% BLOTTO. Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
ARNT interacting protein,hypoxia-inducible factor 1, alpha subunit,hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor),member of PAS superfamily 1