HIF1A polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using HIF1A polyclonal antibody, P564 (Cat # PAB9946) shows detection (left panel) of hydroxylated HIF1A in nuclear extracts of A-549 cells treated with MG132 (a proteosome inhibitor).
Hydroxyproline is not recognized on HIF1A when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation.
Control staining is shown (right panel) using conventional HIF1A polyclonal antibody.
The asterisk marks a band approximately 110 kDa in size corresponding to HIF1A.
The primary antibody was used at a 1 : 1,000 dilution in 2% BLOTTO.
Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HIF1A.
Immunogen
A synthetic peptide corresponding to a region surrounding P564 of human HIF1A.
Host
Rabbit
Reactivity
Bovine, Clawed frog, Dog, Human, Monkey, Mouse, Rat
Specificity
This antibody is directed against human HIF-1alpha HYDROXYP564 and is specific to the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using HIF1A polyclonal antibody, P564 (Cat # PAB9946) shows detection (left panel) of hydroxylated HIF1A in nuclear extracts of A-549 cells treated with MG132 (a proteosome inhibitor).
Hydroxyproline is not recognized on HIF1A when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation.
Control staining is shown (right panel) using conventional HIF1A polyclonal antibody.
The asterisk marks a band approximately 110 kDa in size corresponding to HIF1A.
The primary antibody was used at a 1 : 1,000 dilution in 2% BLOTTO.
Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — HIF1A
Entrez GeneID
3091Protein Accession#
NP_001521;Q16665Gene Name
HIF1A
Gene Alias
HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Gene Description
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Omim ID
603348Gene Ontology
HyperlinkGene Summary
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
ARNT interacting protein|hypoxia-inducible factor 1, alpha subunit|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|member of PAS superfamily 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Hypoxia-inducible factor-1alpha expression in the gastric carcinogenesis sequence and its prognostic role in gastric and gastro-oesophageal adenocarcinomas.
Griffiths EA, Pritchard SA, Valentine HR, Whitchelo N, Bishop PW, Ebert MP, Price PM, Welch IM, West CM.
British Journal of Cancer 2007 Jan; 96(1):95.
Application：IHC-P, Human, Human gastric cancer.
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Mucin 1 oncoprotein blocks hypoxia-inducible factor 1alpha activation in a survival response to hypoxia.
Yin L, Kharbanda S, Kufe D.
The Journal of Biological Chemistry 2007 Jan; 282(1):257.
Application：WB, Human, HCT-116, ZR-75-1 cells.
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IL-4 enhances hypoxia induced HIF-1alpha protein levels in human transformed intestinal cells.
Scharte M, Jurk K, Kehrel B, Zarbock A, Van Aken H, Singbartl K.
FEBS Letters 2006 Nov; 580(27):6399.
Application：WB, Human, HT-29 cells.
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Hypoxia-inducible factor-1alpha expression in the gastric carcinogenesis sequence and its prognostic role in gastric and gastro-oesophageal adenocarcinomas.
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