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Last updated: 2017/12/10
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HIF1A polyclonal antibody

  • Catalog # : PAB9946
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of HIF1A.
  • Immunogen:
  • A synthetic peptide corresponding to a region surrounding P564 of human HIF1A.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Clawed frog, Dog, Human, Monkey, Mouse, Rat
  • Specificity:
  • This antibody is directed against human HIF-1alpha HYDROXYP564 and is specific to the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using HIF1A polyclonal antibody, P564 (Cat # PAB9946) shows detection (left panel) of hydroxylated HIF1A in nuclear extracts of A-549 cells treated with MG132 (a proteosome inhibitor).
    Hydroxyproline is not recognized on HIF1A when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation.
    Control staining is shown (right panel) using conventional HIF1A polyclonal antibody.
    The asterisk marks a band approximately 110 kDa in size corresponding to HIF1A.
    The primary antibody was used at a 1 : 1,000 dilution in 2% BLOTTO.
    Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3091
  • Protein Accession#:
  • NP_001521;Q16665
  • Gene Name:
  • HIF1A
  • Gene Alias:
  • HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
  • Gene Description:
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • Gene Summary:
  • Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • ARNT interacting protein,hypoxia-inducible factor 1, alpha subunit,hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor),member of PAS superfamily 1
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