Western blot analysis is shown using CCND1 polyclonal antibody (Cat # PAB9944) to detect Human CCND1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express CCNB1 and CCND1. Comparison to a molecular weight marker indicates a band of ~34 KDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1 : 500 dilution of the antibody at room temperature. Detection occurred using a 1 : 10,000 of HRP conjugated Goat-anti-Rabbit IgG.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq