CCND1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CCND1.
Immunogen
A synthetic peptide corresponding to human CCND1.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Dog, Frog, Human, Mouse, Orangutan, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis is shown using CCND1 polyclonal antibody (Cat # PAB9944) to detect Human CCND1 present in asynchronous HN30 cell lysates.
HN30 cells, are from head and neck cancer cells that over express CCNB1 and CCND1.
Comparison to a molecular weight marker indicates a band of ~34 KDa corresponding to the expected molecular weight for the protein (arrowhead).
The blot was incubated with a 1 : 500 dilution of the antibody at room temperature.
Detection occurred using a 1 : 10,000 of HRP conjugated Goat-anti-Rabbit IgG.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — CCND1
Entrez GeneID
595Protein Accession#
P24385;NP_444284Gene Name
CCND1
Gene Alias
BCL1, D11S287E, PRAD1, U21B31
Gene Description
cyclin D1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq
Other Designations
B-cell CLL/lymphoma 1|G1/S-specific cyclin D1
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Gonadotropin-inhibiting hormone promotes apoptosis of bovine ovary granulosa cells.
Xu Li, Gaoqing Xu, Zhiqiang Li, Hongyu Liu, Xin Ma, Lianyu Yang, Pengju Zhang, Jing Zhao, Jun Wang, Wenfa Lu.
Life Sciences 2021 Apr; 270:119063.
Application:WB-Ce, Bovine, Bovine granulosa cells.
-
Cyclin D1 expression and cell cycle response in DNA mismatch repair-deficient cells upon methylation and UV-C damage.
Lutzen A, Bisgaard HC, Rasmussen LJ.
Experimental Cell Research 2004 Jan; 292(1):123.
Application:WB, Human, LoVo, SW480 cells.
-
Cyclins and cyclin-dependent kinases: take your partners.
Pines J.
Trends in Biochemical Sciences 1993 Jun; 8(6):195.
Application:IP, WB-Ce, WB-Tr, Human, Mammalian cells, T cells.
-
A novel cyclin encoded by a bcl1-linked candidate oncogene.
Motokura T, Bloom T, Kim HG, Juppner H, Ruderman JV, Kronenberg HM, Arnold A.
Nature 1991 Apr; 350(6318):512.
Application:WB-Ti, Clam, Clam embryos.
-
Gonadotropin-inhibiting hormone promotes apoptosis of bovine ovary granulosa cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com