CIITA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant CIITA.
Immunogen
Recombinant Flag fusion protein corresponding to amino acids 1-333 of human CIITA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
CIITA polyclonal antibody (Cat # PAB9942), generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by immunoblot against lysates of COS-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA.
In panel A, both samples on PVDF were incubated with 10 ug/mL mouse anti-FLAG antibody (Sigma) for 45'.
This blot demonstrates that FLAG-CIITA-336-842 was successfully over-expressed in the Cos-7 cells.
In panel B, both samples on PVDF were incubated with a 1 : 500 dilution of CIITA polyclonal antibody (Cat # PAB9942) for 45'.
The lane containing the lysate of pcDNA3-HA-CIITA transfected cells contains a single band at ~130 kD molecular weight, whereas the lane containing lysate from pcDNA3-FLAG-CIITA-336-842 transfected cells shows no reactivity.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CIITA
Entrez GeneID
4261Gene Name
CIITA
Gene Alias
C2TA, CIITAIV, MHC2TA, NLRA
Gene Description
class II, major histocompatibility complex, transactivator
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. [provided by RefSeq
Other Designations
MHC class II transactivator type III|NLR family, acid domain containing|class II transactivator|nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing
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