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Last updated: 2017/12/10
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CIITA polyclonal antibody

  • Catalog # : PAB9942
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant CIITA.
  • Immunogen:
  • Recombinant Flag fusion protein corresponding to amino acids 1-333 of human CIITA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In antiserum (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • CIITA polyclonal antibody (Cat # PAB9942), generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by immunoblot against lysates of COS-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA.
    In panel A, both samples on PVDF were incubated with 10 ug/mL mouse anti-FLAG antibody (Sigma) for 45'.
    This blot demonstrates that FLAG-CIITA-336-842 was successfully over-expressed in the Cos-7 cells.
    In panel B, both samples on PVDF were incubated with a 1 : 500 dilution of CIITA polyclonal antibody (Cat # PAB9942) for 45'.
    The lane containing the lysate of pcDNA3-HA-CIITA transfected cells contains a single band at ~130 kD molecular weight, whereas the lane containing lysate from pcDNA3-FLAG-CIITA-336-842 transfected cells shows no reactivity.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4261
  • Gene Name:
  • CIITA
  • Gene Alias:
  • C2TA,CIITAIV,MHC2TA,NLRA
  • Gene Description:
  • class II, major histocompatibility complex, transactivator
  • Gene Summary:
  • This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. [provided by RefSeq
  • Other Designations:
  • MHC class II transactivator type III,NLR family, acid domain containing,class II transactivator,nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing
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