Immunoblotting of CDK2 polyclonal antibody (Cat # PAB9940) was used at a 1 : 200 dilution to detect CDK2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 ug of lysate was separated on a 15% SDS-PAGE gel. CDK2 is indicated by an arrowhead as a 32-33 KDa band. Note that multiple isoforms as well as phosphorylated forms of CDK2 may be detected by this antibody. Primary antibody was reacted at room temperature for 1 h. After subsequent washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.
Immunohistochemical staining eith CDK2 polyclonal antibody (Cat # PAB9940) was diluted 1 : 500 to detect CDK2 in human skin tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq
cdc2-related protein kinase,cell devision kinase 2,p33 protein kinase