CDC2 polyclonal antibody (Cat # PAB9939) was used todetect human CDC2 by western blot in untreated (Contol) and drug treated lysates of MCF-7 cells. Lane 1-4 represents 3.1 uM, 6.2 uM, 12.5 uM and 25.0 uM genistein treatment of cells before lysates were prepared. Detection occurs using a 1 : 1,000 dilution. Although this antiserum detects non-specific bands at higher MW, a clear induction ofsignal is observed as the concentration of drug is increased. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
OTTHUMP00000019660,cell cycle controller CDC2,cell division control protein 2 homolog,cell division cycle 2 protein,cyclin-dependent kinase 1,p34 protein kinase