Western blot using CAMK4 polyclonal antibody (Cat # PAB9938) shows detection of a band ~52 KDa corresponding to CAMK4 (arrowhead) in various preparations. Staining ofrat brain lysate is shown in lane 1. Jurkat cell lysate staining is shown in lane 2. Specific reactivity is blocked in both lysates when antibodyis preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CAMK4 was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L]MX for 45 min at room temperature.
Immunohistochemical staining with CAMK4 polyclonal antibody (Cat # PAB9938) was diluted 1 : 500 to detect CAMK4 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq
CAM kinase IV,CAM kinase- GR,brain Ca(2+)-calmodulin-dependent protein kinase type IV,brain Ca++-calmodulin-dependent protein kinase type IV,calcium/calmodulin-dependent protein kinase type IV catalytic chain