Western blot using AP2A1 polyclonal antibody (Cat # PAB9935) shows detection of a band just below 100 KDa correspond-ing to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract. Lane 2 - HeLa. Lane 3 - 293. Lane 4 - A-431. Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/mL of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A1. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP2A1 polyclonal antibody. Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection using a 60-sec exposure time.
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq