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Last updated: 2016/12/4
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AP2A1 polyclonal antibody

  • Catalog # : PAB9935
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of AP2A1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 3-14 of human AP2A1.
  • Host:
  • Goat
  • Reactivity:
  • Chicken, Chimpanzee, Dog, Human, Mouse, Orangutan, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using AP2A1 polyclonal antibody (Cat # PAB9935) shows detection of a band just below 100 KDa correspond-ing to Human AP2A1 in a various preparations.
    Lane 1 - HeLa nuclear extract.
    Lane 2 - HeLa.
    Lane 3 - 293.
    Lane 4 - A-431.
    Lane 5 - Jurkat whole cell lysates.
    In lanes 6-10 the antibody was preincubated with 1 ug/mL of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A1.
    Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP2A1 polyclonal antibody.
    Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.
    A chemiluminescence system was used for signal detection using a 60-sec exposure time.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 160
  • Protein Accession#:
  • O95782;NP_055018(alpha1);NP_036437(alpha2)
  • Gene Name:
  • AP2A1
  • Gene Alias:
  • ADTAA,AP2-ALPHA,CLAPA1
  • Gene Description:
  • adaptor-related protein complex 2, alpha 1 subunit
  • Gene Summary:
  • This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq
  • Other Designations:
  • 100 kDa coated vesicle protein A,adaptin, alpha A,clathrin-associated/assembly/adaptor protein, large, alpha 1
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