AP2A1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AP2A1.
Immunogen
A synthetic peptide corresponding to amino acids 3-14 of human AP2A1.
Host
Goat
Reactivity
Chicken, Chimpanzee, Dog, Human, Mouse, Orangutan, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using AP2A1 polyclonal antibody (Cat # PAB9935) shows detection of a band just below 100 KDa correspond-ing to Human AP2A1 in a various preparations.
Lane 1 - HeLa nuclear extract.
Lane 2 - HeLa.
Lane 3 - 293.
Lane 4 - A-431.
Lane 5 - Jurkat whole cell lysates.
In lanes 6-10 the antibody was preincubated with 1 ug/mL of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A1.
Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of AP2A1 polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection using a 60-sec exposure time.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AP2A1
Entrez GeneID
160Protein Accession#
O95782;NP_055018(alpha1);NP_036437(alpha2)Gene Name
AP2A1
Gene Alias
ADTAA, AP2-ALPHA, CLAPA1
Gene Description
adaptor-related protein complex 2, alpha 1 subunit
Omim ID
601026Gene Ontology
HyperlinkGene Summary
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq
Other Designations
100 kDa coated vesicle protein A|adaptin, alpha A|clathrin-associated/assembly/adaptor protein, large, alpha 1
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Interactome
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Pathway
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Publication Reference
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Identification and regulation of tissue-specific cis-acting elements associated with the human AP-2alpha gene.
Zhang J, Williams T.
Developmental Dynamics 2003 Oct; 228(2):194.
Application:IHC, Mouse, Mouse head.
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Cloning, physical mapping and structural characterization of the human alpha(A)-adaptin gene.
Scorilas A, Levesque MA, Ashworth LK, Diamandis EP.
Gene 2002 May; 289(1-2):191.
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Adaptins: the final recount.
Boehm M, Bonifacino JS.
Molecular Biology of the Cell 2001 Oct; 12(10):2907.
Application:WB-Ti, Mouse, Pig, Brains, Skeletal muscles.
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Identification and regulation of tissue-specific cis-acting elements associated with the human AP-2alpha gene.
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