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Last updated: 2017/8/20
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JUB polyclonal antibody

  • Catalog # : PAB9930
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of JUB.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 224-239 of human JUB.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Dog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:20000-1:80000)
    Western Blot (1:500-1:2500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using JUB polyclonal antibody (Cat # PAB9930) shows detection of a 57 KDa band consistent with the expected MW for JUB (arrowhead).
    Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A-431, 4) Jurkat and 5) 293 whole cell lysates.
    Immunoprecipitation of JUB followed by western blotting may result in cleaner background staining.
    Approximately 5 ug ofeach preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of JUB polyclonal antibody.
    Detection occurred using a 1 : 5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
    Achemiluminescence system was used for signal detection using a 60-sec exposure time.
    Personal Communication. E. Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using JUB polyclonal antibody (Cat # PAB9930) shows detection of JUB-RFP fusion protein in cell lysates (arrow-head).
    Lanes correspond to 1) vector only transfection, 2) human JUB-RFP, 3) mouse JUB-RFP, and 4) mock transfection.
    Approximately 50 ug of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1 : 1,700 dilution of JUB polyclonal antibody.
    Detection occurred using a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nmchannel, green).
    Molecular weight estimation wasmade by comparison to prestained MW markers (indicated at left, 700 nm channel, red).
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q96IF1;NP_116265
  • Gene Name:
  • JUB
  • Gene Alias:
  • Ajuba,MGC15563
  • Gene Description:
  • jub, ajuba homolog (Xenopus laevis)
  • Other Designations:
  • OTTHUMP00000164552,ajuba
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