JUB polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using JUB polyclonal antibody (Cat # PAB9930) shows detection of a 57 KDa band consistent with the expected MW for JUB (arrowhead).
Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A-431, 4) Jurkat and 5) 293 whole cell lysates.
Immunoprecipitation of JUB followed by western blotting may result in cleaner background staining.
Approximately 5 ug ofeach preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of JUB polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
Achemiluminescence system was used for signal detection using a 60-sec exposure time.
Personal Communication. E. Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.
Western Blot (Transfected lysate)
Western blot using JUB polyclonal antibody (Cat # PAB9930) shows detection of JUB-RFP fusion protein in cell lysates (arrow-head).
Lanes correspond to 1) vector only transfection, 2) human JUB-RFP, 3) mouse JUB-RFP, and 4) mock transfection.
Approximately 50 ug of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1 : 1,700 dilution of JUB polyclonal antibody.
Detection occurred using a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nmchannel, green).
Molecular weight estimation wasmade by comparison to prestained MW markers (indicated at left, 700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of JUB.
Immunogen
A synthetic peptide corresponding to amino acids 224-239 of human JUB.
Host
Rabbit
Reactivity
Chimpanzee, Dog, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000-1:80000)
Western Blot (1:500-1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using JUB polyclonal antibody (Cat # PAB9930) shows detection of a 57 KDa band consistent with the expected MW for JUB (arrowhead).
Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A-431, 4) Jurkat and 5) 293 whole cell lysates.
Immunoprecipitation of JUB followed by western blotting may result in cleaner background staining.
Approximately 5 ug ofeach preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of JUB polyclonal antibody.
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
Achemiluminescence system was used for signal detection using a 60-sec exposure time.
Personal Communication. E. Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.Western Blot (Transfected lysate)
Western blot using JUB polyclonal antibody (Cat # PAB9930) shows detection of JUB-RFP fusion protein in cell lysates (arrow-head).
Lanes correspond to 1) vector only transfection, 2) human JUB-RFP, 3) mouse JUB-RFP, and 4) mock transfection.
Approximately 50 ug of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1 : 1,700 dilution of JUB polyclonal antibody.
Detection occurred using a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nmchannel, green).
Molecular weight estimation wasmade by comparison to prestained MW markers (indicated at left, 700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.
IRDye is a trademark of LI-COR, Inc.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — JUB
Entrez GeneID
84962Protein Accession#
Q96IF1;NP_116265Gene Name
JUB
Gene Alias
Ajuba, MGC15563
Gene Description
jub, ajuba homolog (Xenopus laevis)
Omim ID
609066Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000164552|ajuba
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Interactome
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Publication Reference
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Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells.
Hirota T, Kunitoku N, Sasayama T, Marumoto T, Zhang D, Nitta M, Hatakeyama K, Saya H.
Cell 2003 Sep; 114(5):585.
Application：IP-WB, Human, HeLa cells.
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The LIM protein Ajuba is recruited to cadherin-dependent cell junctions through an association with alpha-catenin.
Marie H, Pratt SJ, Betson M, Epple H, Kittler JT, Meek L, Moss SJ, Troyanovsky S, Attwell D, Longmore GD, Braga VM.
The Journal of Biological Chemistry 2003 Jan; 278(2):1220.
Application：IF, WB-Ce, Human, Mouse, MEFs, Keratinocytes, MDA-MB-468 cells.
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Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner.
Goyal RK, Lin P, Kanungo J, Payne AS, Muslin AJ, Longmore GD.
Molecular and Cellular Biology 1999 Jun; 19(6):4379.
Application：WB-Tr, Mouse, ES cells.
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Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells.
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